Human HHEX cDNA Clone in Mammalian Expression Vector
Quick Overview for Human HHEX cDNA Clone in Mammalian Expression Vector (ABIN3317685)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc321626
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HHEX is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Transcription Factors and Medium Suitable for Initiating the Differentiation of Human-Induced Pluripotent Stem Cells to the Hepatocyte Lineage." in: Journal of cellular biochemistry, Vol. 117, Issue 9, pp. 2001-9, (2016) (PubMed).
: "Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAs." in: PLoS ONE, Vol. 9, Issue 6, pp. e100134, (2014) (PubMed).
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: "Transcription Factors and Medium Suitable for Initiating the Differentiation of Human-Induced Pluripotent Stem Cells to the Hepatocyte Lineage." in: Journal of cellular biochemistry, Vol. 117, Issue 9, pp. 2001-9, (2016) (PubMed).
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- HHEX (Hematopoietically Expressed Homeobox (HHEX))
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Alternative Name
- HHEX
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Background
- This gene encodes a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_002729, NP_002720
Target
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