Human HSPA2 cDNA Clone in Mammalian Expression Vector
This is a Heat Shock 70kDa Protein 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3317722
Supplier Product No.: sc319383
$884.07
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HSPA2 cDNA Clone in Mammalian Expression Vector (ABIN3317722)
Gene
HSPA2
(Heat Shock 70kDa Protein 2 (HSPA2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc319383
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HSPA2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The chaperone network connected to human ribosome-associated complex." in: Molecular and cellular biology, Vol. 31, Issue 6, pp. 1160-73, (2011) (PubMed).
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: "The chaperone network connected to human ribosome-associated complex." in: Molecular and cellular biology, Vol. 31, Issue 6, pp. 1160-73, (2011) (PubMed).
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- HSPA2 (Heat Shock 70kDa Protein 2 (HSPA2))
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Alternative Name
- HSPA2
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Background
- In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. [UniProtKB/Swiss-Prot Function]
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NCBI Accession
- NM_021979, NP_068814
Target
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