Human SLC2A9 cDNA Clone in Mammalian Expression Vector
This is a Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 9 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: 1900 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(3 references)
Catalog No. ABIN3318273
Supplier Product No.: sc319239
$472.23
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human SLC2A9 cDNA Clone in Mammalian Expression Vector (ABIN3318273)
Gene
SLC2A9
(Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 9 (SLC2A9))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc319239
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLC2A9 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1900 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Expression of SLC2A9 isoforms in the kidney and their localization in polarized epithelial cells." in: PLoS ONE, Vol. 9, Issue 1, pp. e84996, (2014) (PubMed).
: "Expression, purification, and structural insights for the human uric acid transporter, GLUT9, using the Xenopus laevis oocytes system." in: PLoS ONE, Vol. 9, Issue 10, pp. e108852, (2014) (PubMed).
: "Plasma urate level is directly regulated by a voltage-driven urate efflux transporter URATv1 (SLC2A9) in humans." in: The Journal of biological chemistry, Vol. 283, Issue 40, pp. 26834-8, (2008) (PubMed).
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- SLC2A9 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 9 (SLC2A9))
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Alternative Name
- SLC2A9
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Background
- This gene encodes a member of the SLC2A facilitative glucose transporter family. Members of this family play a significant role in maintaining glucose homeostasis. The encoded protein may play a role in the development and survival of chondrocytes in cartilage matrices. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (2), also known as GLUT9deltaN, contains alternate in-frame segments in the 5' UTR and coding region and uses a different start codon, compared to variant 1. Isoform 2 has a shorter N-terminus, compared to isoform 1.
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NCBI Accession
- NM_001001290, NP_001001290
Target
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