Human SUV39H1 cDNA Clone in Mammalian Expression Vector
This is a Suppressor of Variegation 3-9 Homolog 1 (Drosophila) plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3318350
Supplier Product No.: sc319503
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human SUV39H1 cDNA Clone in Mammalian Expression Vector (ABIN3318350)
Gene
SUV39H1
(Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc319503
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SUV39H1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "A role for SUV39H1-mediated H3K9 trimethylation in the control of genome stability and senescence in WI38 human diploid lung fibroblasts." in: Aging, Vol. 6, Issue 7, pp. 545-63, (2014) (PubMed).
: "SUV39H1 downregulation induces deheterochromatinization of satellite regions and senescence after exposure to ionizing radiation." in: Frontiers in genetics, Vol. 5, pp. 411, (2014) (PubMed).
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- SUV39H1 (Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1))
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Alternative Name
- SUV39H1
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Background
- This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013].Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1.
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NCBI Accession
- NM_003173, NP_003164
Target
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