Human UBA2 cDNA Clone in Mammalian Expression Vector
This is a SUMO1 Activating Enzyme Subunit 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3318441
Supplier Product No.: sc319458
$591.03
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human UBA2 cDNA Clone in Mammalian Expression Vector (ABIN3318441)
Gene
UBA2
(SUMO1 Activating Enzyme Subunit 2 (UBA2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc319458
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human UBA2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: EcoRI-XhoI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Small-molecule activation of SERCA2a SUMOylation for the treatment of heart failure." in: Nature communications, Vol. 6, pp. 7229, (2015) (PubMed).
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: "Small-molecule activation of SERCA2a SUMOylation for the treatment of heart failure." in: Nature communications, Vol. 6, pp. 7229, (2015) (PubMed).
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- UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))
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Alternative Name
- UBA2
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Background
- Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010].
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NCBI Accession
- NM_005499, NP_005490
Target
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