Human AGTR1 cDNA Clone in Mammalian Expression Vector
This is a Angiotensin II Receptor, Type 1 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3318525
Supplier Product No.: sc322091
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human AGTR1 cDNA Clone in Mammalian Expression Vector (ABIN3318525)
Gene
Angiotensin II Type-1 Receptor (AGTR1)
(Angiotensin II Receptor, Type 1 (AGTR1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc322091
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human AGTR1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: KpnI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))
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Alternative Name
- AGTR1
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Background
- Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed, however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012].Transcript Variant: This variant (3), also known as hAT1R-B (PubMed ID:11158334) or hAT1R-C (PubMed ID:16504375), has an alternate exon in the 5' region, which contains an in-frame AUG start codon, compared to variant 1. The resulting isoform (2) has a longer N-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform 2.
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NCBI Accession
- NM_004835, NP_004826
Target
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