Human CYGB cDNA Clone in Mammalian Expression Vector
This is a Cytoglobin plasmid from OriGene - 6 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(6 references)
Catalog No. ABIN3318801
Supplier Product No.: sc321813
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human CYGB cDNA Clone in Mammalian Expression Vector (ABIN3318801)
Gene
CYGB
(Cytoglobin (CYGB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc321813
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CYGB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: RsrII-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Peroxidase activation of cytoglobin by anionic phospholipids: Mechanisms and consequences." in: Biochimica et biophysica acta, Vol. 1861, Issue 5, pp. 391-401, (2016) (PubMed).
: "Cytoglobin ligand binding regulated by changing haem-co-ordination in response to intramolecular disulfide bond formation and lipid interaction." in: The Biochemical journal, Vol. 465, Issue 1, pp. 127-37, (2015) (PubMed).
: "Effect of the distal histidine on the peroxidatic activity of monomeric cytoglobin." in: F1000Research, Vol. 4, pp. 87, (2015) (PubMed).
: "Expression and biological role of cytoglobin in human ovarian cancer." in: Tumour biology, Vol. 35, Issue 7, pp. 6933-9, (2014) (PubMed).
: "DNA damage induced activation of Cygb stabilizes p53 and mediates G1 arrest." in: DNA repair, Vol. 24, pp. 107-12, (2014) (PubMed).
: "Cytoglobin has bimodal: tumour suppressor and oncogene functions in lung cancer cell lines." in: Human molecular genetics, Vol. 22, Issue 16, pp. 3207-17, (2013) (PubMed).
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- CYGB (Cytoglobin (CYGB))
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Alternative Name
- CYGB
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Background
- This gene encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. This gene is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation. [provided by RefSeq, Jan 2012].
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NCBI Accession
- NM_134268, NP_599030
Target
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