Human EPCAM cDNA Clone in Mammalian Expression Vector
This is a Epithelial Cell Adhesion Molecule plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3318861
Supplier Product No.: sc322331
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human EPCAM cDNA Clone in Mammalian Expression Vector (ABIN3318861)
Gene
EpCAM (EPCAM)
(Epithelial Cell Adhesion Molecule (EPCAM))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc322331
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human EPCAM is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: RsrII-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors." in: Nature communications, Vol. 6, pp. 6246, (2015) (PubMed).
: "Epithelial cell adhesion molecule (EpCAM) is associated with prostate cancer metastasis and chemo/radioresistance via the PI3K/Akt/mTOR signaling pathway." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 12, pp. 2736-48, (2013) (PubMed).
: "Ep-CAM is a significant prognostic factor in pancreatic cancer patients by suppressing cell activity." in: Oncogene, Vol. 30, Issue 31, pp. 3468-76, (2011) (PubMed).
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: "Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors." in: Nature communications, Vol. 6, pp. 6246, (2015) (PubMed).
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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Alternative Name
- EPCAM
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Background
- This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008].
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NCBI Accession
- NM_002354, NP_002345
Target
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