Human ORMDL3 cDNA Clone in Mammalian Expression Vector
Quick Overview for Human ORMDL3 cDNA Clone in Mammalian Expression Vector (ABIN3319159)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc321992
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ORMDL3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: RsrII-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "ORMDL3 transgenic mice have increased airway remodeling and airway responsiveness characteristic of asthma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 8, pp. 3475-87, (2014) (PubMed).
: "ORMDL3 is an inducible lung epithelial gene regulating metalloproteases, chemokines, OAS, and ATF6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 41, pp. 16648-53, (2012) (PubMed).
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: "ORMDL3 transgenic mice have increased airway remodeling and airway responsiveness characteristic of asthma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 8, pp. 3475-87, (2014) (PubMed).
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