Human P4HA2 cDNA Clone in Mammalian Expression Vector
This is a Prolyl 4-Hydroxylase, alpha Polypeptide II plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3319163
Supplier Product No.: sc321909
$492.03
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human P4HA2 cDNA Clone in Mammalian Expression Vector (ABIN3319163)
Gene
P4HA2
(Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc321909
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human P4HA2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: RsrII-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- P4HA2 (Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2))
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Alternative Name
- P4HA2
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Background
- This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (2), also known as IIa, contains an alternate in-frame exon, compared to variant 1. Variants 2, 3, and 4 encode isoform 2, which is shorter than isoform 1.
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NCBI Accession
- NM_001017973, NP_001017973
Target
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