Human PRAME cDNA Clone in Mammalian Expression Vector
This is a Preferentially Expressed Antigen in Melanoma plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3319216
Supplier Product No.: sc321921
$470.25
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PRAME cDNA Clone in Mammalian Expression Vector (ABIN3319216)
Gene
PRAME
(Preferentially Expressed Antigen in Melanoma (PRAME))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc321921
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PRAME is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: RsrII-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Molecular Insights on Post-chemotherapy Retinoblastoma by Microarray Gene Expression Analysis." in: Bioinformatics and biology insights, Vol. 7, pp. 289-306, (2013) (PubMed).
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- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
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Alternative Name
- PRAME
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Background
- This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014].Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a).
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NCBI Accession
- NM_206955, NP_996838
Target
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