Human VGF cDNA Clone in Mammalian Expression Vector
This is a VGF Nerve Growth Factor Inducible plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3319480
Supplier Product No.: sc322730
$567.27
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human VGF cDNA Clone in Mammalian Expression Vector (ABIN3319480)
Gene
VGF
(VGF Nerve Growth Factor Inducible (VGF))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc322730
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human VGF is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: RsrII-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Association of promoter methylation of VGF and PGP9.5 with ovarian cancer progression." in: PLoS ONE, Vol. 8, Issue 9, pp. e70878, (2013) (PubMed).
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: "Association of promoter methylation of VGF and PGP9.5 with ovarian cancer progression." in: PLoS ONE, Vol. 8, Issue 9, pp. e70878, (2013) (PubMed).
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- VGF (VGF Nerve Growth Factor Inducible (VGF))
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Alternative Name
- VGF
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Background
- This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_003378, NP_003369
Target
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