Human DAB2 cDNA Clone in Mammalian Expression Vector
This is a Disabled Homolog 2, Mitogen-Responsive phosphoprotein (Drosophila) plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3319739
Supplier Product No.: sc321375
$698.94
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human DAB2 cDNA Clone in Mammalian Expression Vector (ABIN3319739)
Gene
DAB2
(Disabled Homolog 2, Mitogen-Responsive phosphoprotein (Drosophila) (DAB2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc321375
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human DAB2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The histone demethylase Kdm3a is essential to progression through differentiation." in: Nucleic acids research, Vol. 40, Issue 15, pp. 7219-32, (2012) (PubMed).
: "Disabled-2 protein facilitates assembly polypeptide-2-independent recruitment of cystic fibrosis transmembrane conductance regulator to endocytic vesicles in polarized human airway epithelial cells." in: The Journal of biological chemistry, Vol. 287, Issue 18, pp. 15087-99, (2012) (PubMed).
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: "The histone demethylase Kdm3a is essential to progression through differentiation." in: Nucleic acids research, Vol. 40, Issue 15, pp. 7219-32, (2012) (PubMed).
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- DAB2 (Disabled Homolog 2, Mitogen-Responsive phosphoprotein (Drosophila) (DAB2))
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Alternative Name
- DAB2
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Background
- This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).
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NCBI Accession
- NM_001343, NP_001334
Target
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