Human FUS cDNA Clone in Mammalian Expression Vector
This is a Fused in Sarcoma plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3319856
Supplier Product No.: sc320263
$728.64
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human FUS cDNA Clone in Mammalian Expression Vector (ABIN3319856)
Gene
FUS
(Fused in Sarcoma (FUS))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc320263
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human FUS is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "FUS-linked essential tremor associated with motor dysfunction in Drosophila." in: Human genetics, Vol. 135, Issue 11, pp. 1223-1232, (2016) (PubMed).
: "An ALS-associated mutation in the FUS 3'-UTR disrupts a microRNA-FUS regulatory circuitry." in: Nature communications, Vol. 5, pp. 4335, (2014) (PubMed).
: "Roles of long noncoding RNAs in brain development, functional diversification and neurodegenerative diseases." in: Brain research bulletin, Vol. 97, pp. 69-80, (2013) (PubMed).
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- FUS (Fused in Sarcoma (FUS))
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Alternative Name
- FUS
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Target Type
- Viral Protein
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Background
- This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009].Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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NCBI Accession
- NM_004960, NP_004951
Target
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