Human HCLS1 cDNA Clone in Mammalian Expression Vector
Quick Overview for Human HCLS1 cDNA Clone in Mammalian Expression Vector (ABIN3319922)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc321204
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HCLS1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Role of cortactin homolog HS1 in transendothelial migration of natural killer cells." in: PLoS ONE, Vol. 10, Issue 2, pp. e0118153, (2015) (PubMed).
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: "Role of cortactin homolog HS1 in transendothelial migration of natural killer cells." in: PLoS ONE, Vol. 10, Issue 2, pp. e0118153, (2015) (PubMed).
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- HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))
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Alternative Name
- HCLS1
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Background
- Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProtKB/Swiss-Prot Function]Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
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NCBI Accession
- NM_005335, NP_005326
Target
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