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Human HCLS1 cDNA Clone in Mammalian Expression Vector

This is a Hematopoietic Cell-Specific Lyn Substrate 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp. Bacterial selection: Ampicillin.
OriGene
Catalog No. ABIN3319922
Supplier Product No.: sc321204
$679.14
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 4 to 8 Business Days

Quick Overview for Human HCLS1 cDNA Clone in Mammalian Expression Vector (ABIN3319922)

Gene

HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-AC

Promoter

Enhanced CMV Promoter

Bacterial Resistance

Ampicillin

Expression Type

Transient, Stable
  • Species

    Human

    Supplier Product No.

    sc321204

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human HCLS1 is ideal for over-expression of native protein for functional studies.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Selectable Marker

    Neomycin

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Mukherjee, Kim, Mooren, Shahan, Cohan, Cooper: "Role of cortactin homolog HS1 in transendothelial migration of natural killer cells." in: PLoS ONE, Vol. 10, Issue 2, pp. e0118153, (2015) (PubMed).

  • Target

    HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

    Alternative Name

    HCLS1

    Background

    Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProtKB/Swiss-Prot Function]Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).

    NCBI Accession

    NM_005335, NP_005326
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