Human KDM4A cDNA Clone in Mammalian Expression Vector
This is a Lysine (K)-Specific Demethylase 4A plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3319992
Supplier Product No.: sc321310
$917.73
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human KDM4A cDNA Clone in Mammalian Expression Vector (ABIN3319992)
Gene
KDM4A
(Lysine (K)-Specific Demethylase 4A (KDM4A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc321310
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human KDM4A is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Kdm4b histone demethylase is a DNA damage response protein and confers a survival advantage following γ-irradiation." in: The Journal of biological chemistry, Vol. 288, Issue 29, pp. 21376-88, (2013) (PubMed).
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: "Kdm4b histone demethylase is a DNA damage response protein and confers a survival advantage following γ-irradiation." in: The Journal of biological chemistry, Vol. 288, Issue 29, pp. 21376-88, (2013) (PubMed).
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- KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))
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Alternative Name
- KDM4A
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Background
- This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009].
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NCBI Accession
- NM_014663, NP_055478
Target
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