Human POU2F1 cDNA Clone in Mammalian Expression Vector
This is a POU Domain, Class 2, Transcription Factor 1 plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-AC. Insert length: 2500 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3320182
Supplier Product No.: sc321473
$1,011.78
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human POU2F1 cDNA Clone in Mammalian Expression Vector (ABIN3320182)
Gene
POU2F1
(POU Domain, Class 2, Transcription Factor 1 (POU2F1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc321473
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human POU2F1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2500 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." in: Cancer letters, Vol. 368, Issue 1, pp. 115-25, (2015) (PubMed).
: "Distinct interaction of nilotinib and imatinib with P-Glycoprotein in intracellular accumulation and cytotoxicity in CML Cell Line K562 cells." in: Biological & pharmaceutical bulletin, Vol. 37, Issue 8, pp. 1330-5, (2014) (PubMed).
: "Identification of functional amino acid residues involved in polyamine and agmatine transport by human organic cation transporter 2." in: PLoS ONE, Vol. 9, Issue 7, pp. e102234, (2014) (PubMed).
: "The homeobox gene CHX10/VSX2 regulates RdCVF promoter activity in the inner retina." in: Human molecular genetics, Vol. 19, Issue 2, pp. 250-61, (2009) (PubMed).
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- POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))
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Alternative Name
- POU2F1
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Background
- The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010].Transcript Variant: This variant (1) encodes the longest isoform (1).
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NCBI Accession
- NM_002697, NP_002688
Target
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