Human TRPV2 cDNA Clone in Mammalian Expression Vector
Quick Overview for Human TRPV2 cDNA Clone in Mammalian Expression Vector (ABIN3320407)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
-
-
Species
- Human
-
Supplier Product No.
- sc320356
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human TRPV2 is ideal for over-expression of native protein for functional studies.
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Selectable Marker
- Neomycin
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "Activation and inhibition of thermosensitive TRP channels by voacangine, an alkaloid present in Voacanga africana, an African tree." in: Journal of natural products, Vol. 77, Issue 2, pp. 285-97, (2014) (PubMed).
: "Sulphur-containing compounds of durian activate the thermogenesis-inducing receptors TRPA1 and TRPV1." in: Food chemistry, Vol. 157, pp. 213-20, (2014) (PubMed).
: "Conserved residues within the putative S4-S5 region serve distinct functions among thermosensitive vanilloid transient receptor potential (TRPV) channels." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41455-62, (2010) (PubMed).
-
-
- TRPV2 (Transient Receptor Potential Cation Channel, Subfamily V, Member 2 (TRPV2))
-
Alternative Name
- TRPV2
-
Background
- This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq, Jul 2008].
-
NCBI Accession
- NM_016113, NP_057197
Target
-