Human NR1H4 cDNA Clone in Mammalian Expression Vector
This is a Nuclear Receptor Subfamily 1, Group H, Member 4 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Neo. Insert length: not_set. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3376525
Supplier Product No.: sc329876
$724.68
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human NR1H4 cDNA Clone in Mammalian Expression Vector (ABIN3376525)
Gene
NR1H4
(Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Neo
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc329876
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NR1H4 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: SgfI-MluI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))
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Alternative Name
- NR1H4
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Background
- This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016].Transcript Variant: This variant (4) contains an alternate 5' terminal exon, and uses an alternate in-frame donor splice site at an internal coding exon compared to variant 1. This results in translation initiation from an alternate in-frame start codon, and a longer isoform (4, also known as FXRbeta-) with a distinct N-terminus and missing a 4 aa protein segment compared to isoform 1.
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NCBI Accession
- NM_001206992, NP_001193921
Target
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