Human ARHGEF18 cDNA Clone in Mammalian Expression Vector
This is a rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Neo. Insert length: 3000 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3376554
Supplier Product No.: sc308747
$875.16
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ARHGEF18 cDNA Clone in Mammalian Expression Vector (ABIN3376554)
Gene
ARHGEF18
(rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Neo
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- sc308747
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ARHGEF18 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3000 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
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Alternative Name
- ARHGEF18
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Background
- Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008].Transcript Variant: This variant (1) represents the shorter transcript, and encodes the shorter isoform (a).
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NCBI Accession
- NM_015318, NP_056133
Target
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