Human ORAI3 cDNA Clone in Mammalian Expression Vector
This is a ORAI Calcium Release-Activated Calcium Modulator 3 plasmid from OriGene - 5 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2400 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(5 references)
Catalog No. ABIN3376788
Supplier Product No.: sc111493
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ORAI3 cDNA Clone in Mammalian Expression Vector (ABIN3376788)
Gene
ORAI3
(ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc111493
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ORAI3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: ECoRI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2400 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Antibody-mediated targeting of the Orai1 calcium channel inhibits T cell function." in: PLoS ONE, Vol. 8, Issue 12, pp. e82944, (2013) (PubMed).
: "Store-dependent and -independent modes regulating Ca2+ release-activated Ca2+ channel activity of human Orai1 and Orai3." in: The Journal of biological chemistry, Vol. 283, Issue 25, pp. 17662-71, (2008) (PubMed).
: "Orai proteins interact with TRPC channels and confer responsiveness to store depletion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 11, pp. 4682-7, (2007) (PubMed).
: "Calcium inhibition and calcium potentiation of Orai1, Orai2, and Orai3 calcium release-activated calcium channels." in: The Journal of biological chemistry, Vol. 282, Issue 24, pp. 17548-56, (2007) (PubMed).
: "Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24979-90, (2006) (PubMed).
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