Human POR cDNA Clone in Mammalian Expression Vector
This is a P450 (Cytochrome) Oxidoreductase plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2650 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3376799
Supplier Product No.: sc100401
$617.76
Plus shipping costs $50.00
10 μg
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Quick Overview for Human POR cDNA Clone in Mammalian Expression Vector (ABIN3376799)
Gene
POR
(P450 (Cytochrome) Oxidoreductase (POR))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc100401
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human POR is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: ECoRI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2650 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Identification and characterization of a novel CYP2C9 allelic variant in a warfarin-sensitive patient." in: Pharmacogenomics, Vol. 16, Issue 13, pp. 1475-86, (2015) (PubMed).
: "MicroRNA-205 suppresses the oral carcinoma oncogenic activity via down-regulation of Axin-2 in KB human oral cancer cell." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 71-9, (2014) (PubMed).
: "In vitro functional characterization of 37 CYP2C9 allelic isoforms found in Chinese Han population." in: Acta pharmacologica Sinica, Vol. 34, Issue 11, pp. 1449-56, (2013) (PubMed).
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- POR (P450 (Cytochrome) Oxidoreductase (POR))
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Alternative Name
- POR
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Background
- This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_000941, NP_000932
Target
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