Human ACSL4 cDNA Clone in Mammalian Expression Vector
This is a Acyl-CoA Synthetase Long-Chain Family Member 4 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 5230 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3376896
Supplier Product No.: sc109208
$645.48
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ACSL4 cDNA Clone in Mammalian Expression Vector (ABIN3376896)
Gene
ACSL4
(Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc109208
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ACSL4 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5230 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Identification of ACSL4 as a biomarker and contributor of ferroptosis." in: Biochemical and biophysical research communications, Vol. 478, Issue 3, pp. 1338-43, (2016) (PubMed).
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- ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))
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Alternative Name
- ACSL4
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Background
- The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016].Transcript Variant: This variant (2) has an additional segment in the 5' region, which introduces an earlier in-frame start codon, as compared to variant 1. The resulting isoform (2) has a 41 aa longer hydrophobic N-terminus, which may impact localization, as compared to isoform 1.
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NCBI Accession
- NM_022977, NP_075266
Target
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