Human AKR1D1 cDNA Clone in Mammalian Expression Vector
This is a Aldo-Keto Reductase Family 1, Member D1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2750 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3376977
Supplier Product No.: sc116410
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human AKR1D1 cDNA Clone in Mammalian Expression Vector (ABIN3376977)
Gene
AKR1D1
(Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116410
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human AKR1D1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2750 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Genetic variation in aldo-keto reductase 1D1 (AKR1D1) affects the expression and activity of multiple cytochrome P450s." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 41, Issue 8, pp. 1538-47, (2013) (PubMed).
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: "Genetic variation in aldo-keto reductase 1D1 (AKR1D1) affects the expression and activity of multiple cytochrome P450s." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 41, Issue 8, pp. 1538-47, (2013) (PubMed).
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- AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
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Alternative Name
- AKR1D1
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Background
- The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010].Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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NCBI Accession
- NM_005989, NP_005980
Target
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