Human AMOTL2 cDNA Clone in Mammalian Expression Vector
This is a Angiomotin Like 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 3460 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3377005
Supplier Product No.: sc114418
$1,388.97
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human AMOTL2 cDNA Clone in Mammalian Expression Vector (ABIN3377005)
Gene
AMOTL2
(Angiomotin Like 2 (AMOTL2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc114418
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human AMOTL2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3460 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Actin-binding and cell proliferation activities of angiomotin family members are regulated by Hippo pathway-mediated phosphorylation." in: The Journal of biological chemistry, Vol. 288, Issue 52, pp. 37296-307, (2013) (PubMed).
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- AMOTL2 (Angiomotin Like 2 (AMOTL2))
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Alternative Name
- AMOTL2
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Background
- Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013].Transcript Variant: This variant (2) differs in the 5' UTR, uses an alternate in-frame splice site in the 3' coding region and initiates translation at a downstream start site, compared to variant 1. It encodes isoform 2, which is shorter at the N-terminus and contains an additional internal amino acid compared to isoform 1.
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NCBI Accession
- NM_016201, NP_057285
Target
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