Human ARHGAP32 cDNA Clone in Mammalian Expression Vector
This is a rho GTPase Activating Protein 32 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 7070 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3377068
Supplier Product No.: sc114870
$1,446.39
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ARHGAP32 cDNA Clone in Mammalian Expression Vector (ABIN3377068)
Gene
ARHGAP32
(rho GTPase Activating Protein 32 (ARHGAP32))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc114870
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ARHGAP32 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 7070 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- ARHGAP32 (rho GTPase Activating Protein 32 (ARHGAP32))
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Alternative Name
- ARHGAP32
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Background
- RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA, MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008].Transcript Variant: This variant (2) lacks multiple exons in the 5' UTR and 5' coding region and has a unique exon for its 5' UTR, compared to variant 1. These differences cause translation initiation from a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus which lacks phox homology and src homology 3 domains, compared to isoform 1.
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NCBI Accession
- NM_014715, NP_055530
Target
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