Human BMP4 cDNA Clone in Mammalian Expression Vector
This is a Bone Morphogenetic Protein 4 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1730 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3377191
Supplier Product No.: sc108985
$452.43
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human BMP4 cDNA Clone in Mammalian Expression Vector (ABIN3377191)
Gene
BMP4
(Bone Morphogenetic Protein 4 (BMP4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc108985
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human BMP4 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1730 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
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Alternative Name
- BMP4
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Background
- This gene encodes a member of the bone morphogenetic protein (BMP) family of proteins, which is part of the transforming growth factor-beta (TGF-beta) superfamily. Members of the BMP family play an important role in bone and cartilage development. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015].Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
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NCBI Accession
- NM_001202, NP_001193
Target
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