Human CRKL cDNA Clone in Mammalian Expression Vector
This is a V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1910 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(4 references)
Catalog No. ABIN3377578
Supplier Product No.: sc116881
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human CRKL cDNA Clone in Mammalian Expression Vector (ABIN3377578)
Gene
CrkL (CRKL)
(V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116881
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CRKL is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1910 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "CRKL promotes lung cancer cell invasion through ERK-MMP9 pathway." in: Molecular carcinogenesis, Vol. 54 Suppl 1, pp. E35-44, (2015) (PubMed).
: "CRKL protein overexpression enhances cell proliferation and invasion in pancreatic cancer." in: Tumour biology, Vol. 36, Issue 2, pp. 1015-22, (2015) (PubMed).
: "Clinical significance and biological roles of CRKL in human bladder carcinoma." in: Tumour biology, Vol. 35, Issue 5, pp. 4101-6, (2014) (PubMed).
: "Genomic and functional analysis identifies CRKL as an oncogene amplified in lung cancer." in: Oncogene, Vol. 29, Issue 10, pp. 1421-30, (2010) (PubMed).
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- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
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Alternative Name
- CRKL
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Background
- This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009].
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NCBI Accession
- NM_005207, NP_005198
Target
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