Human DICER1 cDNA Clone in Mammalian Expression Vector
This is a Dicer 1, Ribonuclease Type III plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 7500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3377741
Supplier Product No.: sc110307
$2,398.77
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human DICER1 cDNA Clone in Mammalian Expression Vector (ABIN3377741)
Gene
DICER1
(Dicer 1, Ribonuclease Type III (DICER1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc110307
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human DICER1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 7500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Human Dicer binds short single-strand and double-strand RNA with high affinity and interacts with different regions of the nucleic acids." in: The Journal of biological chemistry, Vol. 284, Issue 4, pp. 2535-48, (2009) (PubMed).
: "Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells." in: Nucleic acids research, Vol. 35, Issue 19, pp. 6598-610, (2007) (PubMed).
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- DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
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Alternative Name
- DICER1
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Background
- This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010].Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, 4 and 5 encode the same isoform (1).
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NCBI Accession
- NM_030621, NP_085124
Target
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