Human EXO1 cDNA Clone in Mammalian Expression Vector
This is a Exonuclease 1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 3300 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3377942
Supplier Product No.: sc124403
$768.24
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human EXO1 cDNA Clone in Mammalian Expression Vector (ABIN3377942)
Gene
Exonuclease 1 (EXO1)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc124403
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human EXO1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3300 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Phosphorylation of Exo1 modulates homologous recombination repair of DNA double-strand breaks." in: Nucleic acids research, Vol. 38, Issue 6, pp. 1821-31, (2010) (PubMed).
: "Involvement of Exo1b in DNA damage-induced apoptosis." in: Nucleic acids research, Vol. 37, Issue 10, pp. 3452-63, (2009) (PubMed).
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- Exonuclease 1 (EXO1)
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Alternative Name
- EXO1
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Background
- This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) encodes the longer isoform (b).
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NCBI Accession
- NM_006027, NP_006018
Target
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