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Human FABP1 cDNA Clone in Mammalian Expression Vector

This is a Fatty Acid Binding Protein 1, Liver plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 700 bp. Transient expression. Suitable for PExp. Bacterial selection: Ampicillin.
OriGene
Catalog No. ABIN3377958
Supplier Product No.: sc119222
$148.50
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 4 to 8 Business Days

Quick Overview for Human FABP1 cDNA Clone in Mammalian Expression Vector (ABIN3377958)

Gene

FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-XL4

Promoter

Enhanced CMV Promoter, T7 Promoter

Bacterial Resistance

Ampicillin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc119222

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human FABP1 is ideal for over-expression of native protein for functional studies.

    Specificity

    Restriction Site: NotI-NotI

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    700 bp

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Rowland, Hallifax, Nussio, Shapter, Mackenzie, Brian Houston, Knights, Miners: "Characterization of the comparative drug binding to intra- (liver fatty acid binding protein) and extra- (human serum albumin) cellular proteins." in: Xenobiotica; the fate of foreign compounds in biological systems, Vol. 45, Issue 10, pp. 847-57, (2015) (PubMed).

    Martin, McIntosh, Huang, Gupta, Atshaves, Landrock, Landrock, Kier, Schroeder: "The human liver fatty acid binding protein T94A variant alters the structure, stability, and interaction with fibrates." in: Biochemistry, Vol. 52, Issue 51, pp. 9347-57, (2013) (PubMed).

  • Target

    FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))

    Alternative Name

    FABP1

    Background

    This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011].

    NCBI Accession

    NM_001443, NP_001434
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