Human FBXO21 cDNA Clone in Mammalian Expression Vector
This is a F-Box Protein 21 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3378018
Supplier Product No.: sc124348
$579.15
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human FBXO21 cDNA Clone in Mammalian Expression Vector (ABIN3378018)
Gene
FBXO21
(F-Box Protein 21 (FBXO21))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc124348
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human FBXO21 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2900 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 50, pp. 15372-7, (2015) (PubMed).
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- FBXO21 (F-Box Protein 21 (FBXO21))
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Alternative Name
- FBXO21
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Background
- This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) contains an additional 21 nt fragment within the coding region, and thus encodes a 7 aa longer isoform than variant 2.
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NCBI Accession
- NM_033624, NP_296373
Target
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