Human FOXO1 cDNA Clone in Mammalian Expression Vector
This is a Forkhead Box O1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3378090
Supplier Product No.: sc118890
$906.84
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human FOXO1 cDNA Clone in Mammalian Expression Vector (ABIN3378090)
Gene
FOXO1
(Forkhead Box O1 (FOXO1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118890
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human FOXO1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The antiapoptotic function of miR-96 in prostate cancer by inhibition of FOXO1." in: PLoS ONE, Vol. 8, Issue 11, pp. e80807, (2013) (PubMed).
: "The transcription of FOXO genes is stimulated by FOXO3 and repressed by growth factors." in: The Journal of biological chemistry, Vol. 284, Issue 16, pp. 10334-42, (2009) (PubMed).
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- FOXO1 (Forkhead Box O1 (FOXO1))
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Alternative Name
- FOXO1
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Background
- This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_002015, NP_002006
Target
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