Human Fzd1 cDNA Clone in Mammalian Expression Vector
This is a Frizzled Family Receptor 1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4300 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(3 references)
Catalog No. ABIN3378121
Supplier Product No.: sc117910
$596.97
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human Fzd1 cDNA Clone in Mammalian Expression Vector (ABIN3378121)
Gene
FZD1 (Fzd1)
(Frizzled Family Receptor 1 (Fzd1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117910
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human FZD1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4300 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "β-Catenin-dependent pathway activation by both promiscuous "canonical" WNT3a-, and specific "noncanonical" WNT4- and WNT5a-FZD receptor combinations with strong differences in LRP5 and LRP6 ..." in: Cellular signalling, Vol. 26, Issue 2, pp. 260-7, (2013) (PubMed).
: "Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer." in: British journal of cancer, Vol. 100, Issue 7, pp. 1165-74, (2009) (PubMed).
: "R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 37, pp. 14700-5, (2007) (PubMed).
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- FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))
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Alternative Name
- FZD1
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Background
- Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_003505, NP_003496
Target
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