Human ID4 cDNA Clone in Mammalian Expression Vector
cDNA
PExp
Mammalian Expression Vector
pCMV6-XL4
Ampicillin
Enhanced CMV Promoter
unconjugated
Transient
1 image
(2 references)
Catalog No. ABIN3378425
Supplier Product No.: sc127564
Quick Overview for Human ID4 cDNA Clone in Mammalian Expression Vector (ABIN3378425)
Gene
ID4
(Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc127564
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ID4 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3100 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "miRNA-342 Regulates CEACAM1-induced Lumen Formation in a Three-dimensional Model of Mammary Gland Morphogenesis." in: The Journal of biological chemistry, Vol. 291, Issue 32, pp. 16777-86, (2016) (PubMed).
: "Induction of Lumen Formation in a Three-dimensional Model of Mammary Morphogenesis by Transcriptional Regulator ID4: ROLE OF CaMK2D IN THE EPIGENETIC REGULATION OF ID4 GENE EXPRESSION." in: The Journal of biological chemistry, Vol. 291, Issue 32, pp. 16766-76, (2016) (PubMed).
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: "miRNA-342 Regulates CEACAM1-induced Lumen Formation in a Three-dimensional Model of Mammary Gland Morphogenesis." in: The Journal of biological chemistry, Vol. 291, Issue 32, pp. 16777-86, (2016) (PubMed).
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- ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))
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Alternative Name
- ID4
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Background
- This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011].
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NCBI Accession
- NM_001546, NP_001537
Target
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