Human IGFBP7 cDNA Clone in Mammalian Expression Vector
This is a Insulin-Like Growth Factor Binding Protein 7 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1200 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3378439
Supplier Product No.: sc119176
$445.50
Plus shipping costs $50.00
10 μg
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Quick Overview for Human IGFBP7 cDNA Clone in Mammalian Expression Vector (ABIN3378439)
Gene
IGFBP7
(Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119176
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human IGFBP7 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1200 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "IGFBP7 induces apoptosis of acute myeloid leukemia cells and synergizes with chemotherapy in suppression of leukemia cell survival." in: Cell death & disease, Vol. 5, pp. e1300, (2014) (PubMed).
: "Insulin-like growth factor-binding protein-7 functions as a potential tumor suppressor in hepatocellular carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 21, pp. 6693-701, (2011) (PubMed).
: "Insulin-like growth factor-binding protein 7 alters the sensitivity to interferon-based anticancer therapy in hepatocellular carcinoma cells." in: British journal of cancer, Vol. 102, Issue 10, pp. 1483-90, (2010) (PubMed).
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- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
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Alternative Name
- IGFBP7
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Background
- This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011].Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).
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NCBI Accession
- NM_001553, NP_001544
Target
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