Human JAK1 cDNA Clone in Mammalian Expression Vector
This is a Janus Kinase 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 5100 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3378511
Supplier Product No.: sc118761
$1,477.08
Plus shipping costs $50.00
10 μg
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Quick Overview for Human JAK1 cDNA Clone in Mammalian Expression Vector (ABIN3378511)
Gene
JAK1
(Janus Kinase 1 (JAK1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118761
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human JAK1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5100 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Identification of somatic JAK1 mutations in patients with acute myeloid leukemia." in: Blood, Vol. 111, Issue 9, pp. 4809-12, (2008) (PubMed).
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- JAK1 (Janus Kinase 1 (JAK1))
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Alternative Name
- JAK1
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Background
- Janus kinase 1, is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016].
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NCBI Accession
- NM_002227, NP_002218
Target
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