Human KCNJ10 cDNA Clone in Mammalian Expression Vector
This is a Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(3 references)
Catalog No. ABIN3378540
Supplier Product No.: sc118741
$452.43
Plus shipping costs $50.00
10 μg
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Quick Overview for Human KCNJ10 cDNA Clone in Mammalian Expression Vector (ABIN3378540)
Gene
KCNJ10
(Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118741
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human KCNJ10 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Superresolution Imaging of Aquaporin-4 Cluster Size in Antibody-Stained Paraffin Brain Sections." in: Biophysical journal, Vol. 109, Issue 12, pp. 2511-22, (2015) (PubMed).
: "Development of a selective small-molecule inhibitor of Kir1.1, the renal outer medullary potassium channel." in: Molecular pharmacology, Vol. 79, Issue 1, pp. 42-50, (2010) (PubMed).
: "High-throughput screening reveals a small-molecule inhibitor of the renal outer medullary potassium channel and Kir7.1." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 1094-103, (2009) (PubMed).
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- KCNJ10 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10))
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Alternative Name
- KCNJ10
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Background
- This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_002241, NP_002232
Target
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