Human KLF2 cDNA Clone in Mammalian Expression Vector
This is a Kruppel-Like Factor 2 (Lung) plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1720 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3378605
Supplier Product No.: sc127849
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human KLF2 cDNA Clone in Mammalian Expression Vector (ABIN3378605)
Gene
KLF2
(Kruppel-Like Factor 2 (Lung) (KLF2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc127849
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human KLF2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1720 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The Krüppel-like factor 2 transcription factor gene is recurrently mutated in splenic marginal zone lymphoma. ..." in: Leukemia, Vol. 29, Issue 2, pp. 503-7, (2015) (PubMed).
: "FOXO1 impairs whereas statin protects endothelial function in diabetes through reciprocal regulation of Kruppel-like factor 2." in: Cardiovascular research, Vol. 97, Issue 1, pp. 143-52, (2012) (PubMed).
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- KLF2 (Kruppel-Like Factor 2 (Lung) (KLF2))
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Alternative Name
- KLF2
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Background
- Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1, MIM 601974) and the selectin CD62L (SELL, MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011].
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NCBI Accession
- NM_016270, NP_057354
Target
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