Human MINA cDNA Clone in Mammalian Expression Vector
This is a MYC Induced Nuclear Antigen plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3378907
Supplier Product No.: sc103492
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MINA cDNA Clone in Mammalian Expression Vector (ABIN3378907)
Gene
MINA
(MYC Induced Nuclear Antigen (MINA))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc103492
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MINA is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- MINA (MYC Induced Nuclear Antigen (MINA))
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Alternative Name
- MINA
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Background
- MINA is a c-Myc (MYC, MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391], Zhang et al., 2005 [PubMed 15897898]).[supplied by OMIM, May 2008].Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform B), compared to isoform A. Variants 3 and 4 encode the same protein (isoform B).
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NCBI Accession
- NM_032778, NP_116167
Target
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