Human MMP16 cDNA Clone in Mammalian Expression Vector
This is a Matrix Metallopeptidase 16 (Membrane-inserted) plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4110 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3378927
Supplier Product No.: sc316625
$560.34
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MMP16 cDNA Clone in Mammalian Expression Vector (ABIN3378927)
Gene
MMP16
(Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc316625
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MMP16 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4110 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Biochemical characterization and zinc binding group (ZBGs) inhibition studies on the catalytic domain of MMP7 (cdMMP7)." in: Journal of inorganic biochemistry, Vol. 165, pp. 7-17, (2016) (PubMed).
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- MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))
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Alternative Name
- MMP16
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Background
- Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010].Transcript Variant: This variant (1) encodes a membrane-bound form of matrix metalloproteinase 16 (isoform 1). This protein has a longer C-terminus, as compared to isoform 2, contains a transmembrane domain and is expressed at the cell surface. Studies in rats suggest that it may be involved in matrix remodeling in blood vessels.
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NCBI Accession
- NM_005941, NP_005932
Target
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