Human MYCN cDNA Clone in Mammalian Expression Vector
This is a N-Myc Proto-Oncogene Protein plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(4 references)
Catalog No. ABIN3379034
Supplier Product No.: sc116780
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MYCN cDNA Clone in Mammalian Expression Vector (ABIN3379034)
Gene
MYCN
(N-Myc Proto-Oncogene Protein (MYCN))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116780
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MYCN is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Polyamine metabolism is sensitive to glycolysis inhibition in human neuroblastoma cells." in: The Journal of biological chemistry, Vol. 290, Issue 10, pp. 6106-19, (2015) (PubMed).
: "Inhibition of autophagy potentiates the efficacy of Gli inhibitor GANT-61 in MYCN-amplified neuroblastoma cells." in: BMC cancer, Vol. 14, pp. 768, (2014) (PubMed).
: "Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 44, pp. 18048-53, (2012) (PubMed).
: "N-myc is a novel regulator of PI3K-mediated VEGF expression in neuroblastoma." in: Oncogene, Vol. 27, Issue 28, pp. 3999-4007, (2008) (PubMed).
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- MYCN (N-Myc Proto-Oncogene Protein (MYCN))
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Alternative Name
- MYCN
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Background
- This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014].Transcript Variant: This variant (2) lacks segment 1b in the 5' region, compared to variant 1. This variant includes two open reading frames, the isoform 1 represented by this RefSeq is translated from the downstream open reading frame. This transcript and variant 1 encode the same isoform 1.
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NCBI Accession
- NM_005378, NP_005369
Target
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