Human NOS2 cDNA Clone in Mammalian Expression Vector
This is a Nitric Oxide Synthase 2, Inducible plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4100 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3379141
Supplier Product No.: sc300111
$1,490.94
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human NOS2 cDNA Clone in Mammalian Expression Vector (ABIN3379141)
Gene
NOS2
(Nitric Oxide Synthase 2, Inducible (NOS2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc300111
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NOS2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4100 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Microsomal PGE2 synthase-1 regulates melanoma cell survival and associates with melanoma disease progression." in: Pigment cell & melanoma research, Vol. 29, Issue 3, pp. 297-308, (2016) (PubMed).
: "C. pneumoniae disrupts eNOS trafficking and impairs NO production in human aortic endothelial cells." in: Cellular microbiology, Vol. 17, Issue 1, pp. 119-30, (2014) (PubMed).
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- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
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Alternative Name
- NOS2
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Background
- Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein.
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NCBI Accession
- NM_000625, NP_000616
Target
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