Human NPR2 cDNA Clone in Mammalian Expression Vector
This is a Natriuretic Peptide Receptor B/guanylate Cyclase B (Atrionatriuretic Peptide Receptor B) plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 3300 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3379152
Supplier Product No.: sc117646
$1,354.32
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human NPR2 cDNA Clone in Mammalian Expression Vector (ABIN3379152)
Gene
NPR2
(Natriuretic Peptide Receptor B/guanylate Cyclase B (Atrionatriuretic Peptide Receptor B) (NPR2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117646
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NPR2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3300 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Competitive binding of musclin to natriuretic peptide receptor 3 with atrial natriuretic peptide." in: The Journal of endocrinology, Vol. 201, Issue 2, pp. 287-95, (2009) (PubMed).
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- NPR2 (Natriuretic Peptide Receptor B/guanylate Cyclase B (Atrionatriuretic Peptide Receptor B) (NPR2))
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Alternative Name
- NPR2
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Background
- This gene encodes natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. The protein is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. Mutations in this gene are the cause of acromesomelic dysplasia Maroteaux type. [provided by RefSeq, Jul 2008].Transcript Variant: This NPR2 isoform (b) does not include the alternate exon found in isoform a, and thus isoform b contains a different, and longer, carboxy-terminus.
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NCBI Accession
- NM_003995, NP_003986
Target
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