Human RB1 cDNA Clone in Mammalian Expression Vector
This is a Retinoblastoma 1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3379721
Supplier Product No.: sc119971
$1,263.24
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human RB1 cDNA Clone in Mammalian Expression Vector (ABIN3379721)
Gene
Retinoblastoma 1 (RB1)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119971
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RB1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "A Point Mutation in DNA Polymerase β (POLB) Gene Is Associated with Increased Progesterone Receptor (PR) Expression and Intraperitoneal Metastasis in Gastric Cancer." in: Journal of Cancer, Vol. 7, Issue 11, pp. 1472-80, (2016) (PubMed).
: "Cyclin-dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis." in: The Journal of clinical investigation, Vol. 124, Issue 3, pp. 1083-97, (2014) (PubMed).
: "IRF8 suppresses pathological cardiac remodelling by inhibiting calcineurin signalling." in: Nature communications, Vol. 5, pp. 3303, (2014) (PubMed).
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: "A Point Mutation in DNA Polymerase β (POLB) Gene Is Associated with Increased Progesterone Receptor (PR) Expression and Intraperitoneal Metastasis in Gastric Cancer." in: Journal of Cancer, Vol. 7, Issue 11, pp. 1472-80, (2016) (PubMed).
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- Retinoblastoma 1 (RB1)
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Alternative Name
- RB1
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Background
- The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_000321, NP_000312
Target
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