Human RETN cDNA Clone in Mammalian Expression Vector
This is a Resistin plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 580 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3379758
Supplier Product No.: sc113077
$222.75
Plus shipping costs $50.00
10 μg
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Quick Overview for Human RETN cDNA Clone in Mammalian Expression Vector (ABIN3379758)
Gene
Resistin (RETN)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc113077
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RETN is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 580 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Identification and functional characterization of a missense mutation in resistin in two patients with severe obesity and insulin resistance." in: European journal of endocrinology, Vol. 164, Issue 6, pp. 927-36, (2011) (PubMed).
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: "Identification and functional characterization of a missense mutation in resistin in two patients with severe obesity and insulin resistance." in: European journal of endocrinology, Vol. 164, Issue 6, pp. 927-36, (2011) (PubMed).
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- Resistin (RETN)
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Alternative Name
- RETN
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Background
- This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010].Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein.
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NCBI Accession
- NM_020415, NP_065148
Target
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