Human REV1 cDNA Clone in Mammalian Expression Vector
This is a REV1, Polymerase (DNA Directed) plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4860 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3379759
Supplier Product No.: sc114312
$1,079.10
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human REV1 cDNA Clone in Mammalian Expression Vector (ABIN3379759)
Gene
REV1
(REV1, Polymerase (DNA Directed) (REV1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc114312
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human REV1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4860 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Two distinct translesion synthesis pathways across a lipid peroxidation-derived DNA adduct in mammalian cells." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 191-8, (2008) (PubMed).
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: "Two distinct translesion synthesis pathways across a lipid peroxidation-derived DNA adduct in mammalian cells." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 191-8, (2008) (PubMed).
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- REV1 (REV1, Polymerase (DNA Directed) (REV1))
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Alternative Name
- REV1
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Background
- This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016].Transcript Variant: This variant (1) encodes the longer protein (isoform 1).
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NCBI Accession
- NM_016316, NP_057400
Target
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