Human RLIM cDNA Clone in Mammalian Expression Vector
This is a Ring Finger Protein, LIM Domain Interacting plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 6510 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3379794
Supplier Product No.: sc110429
$576.18
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human RLIM cDNA Clone in Mammalian Expression Vector (ABIN3379794)
Gene
RLIM
(Ring Finger Protein, LIM Domain Interacting (RLIM))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc110429
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RLIM is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 6510 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Regulation of estrogen-dependent transcription by the LIM cofactors CLIM and RLIM in breast cancer." in: Cancer research, Vol. 69, Issue 1, pp. 128-36, (2009) (PubMed).
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: "Regulation of estrogen-dependent transcription by the LIM cofactors CLIM and RLIM in breast cancer." in: Cancer research, Vol. 69, Issue 1, pp. 128-36, (2009) (PubMed).
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- RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))
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Alternative Name
- RLIM
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Background
- The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009].Transcript Variant: This variant (1) lacks an alternate exon in the 5' UTR, compared to variant 2, and may represent the more abundant form. Both variants encode the same protein.
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NCBI Accession
- NM_016120, NP_057204
Target
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