Human SLCO1B1 cDNA Clone in Mammalian Expression Vector
This is a Solute Carrier Organic Anion Transporter Family, Member 1B1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3380066
Supplier Product No.: sc116071
$940.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human SLCO1B1 cDNA Clone in Mammalian Expression Vector (ABIN3380066)
Gene
SLCO1B1
(Solute Carrier Organic Anion Transporter Family, Member 1B1 (SLCO1B1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc116071
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human SLCO1B1 is ideal for over-expression of native protein for functional studies.
-
Specificity
- Restriction Site: NotI-NotI
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Insert Length
- 2800 bp
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "Role of OATP transporters in steroid uptake by prostate cancer cells in vivo." in: Prostate cancer and prostatic diseases, (2016) (PubMed).
: "Rare versus common variants in pharmacogenetics: SLCO1B1 variation and methotrexate disposition." in: Genome research, Vol. 22, Issue 1, pp. 1-8, (2012) (PubMed).
: "Mechanisms of pH-gradient driven transport mediated by organic anion polypeptide transporters." in: American journal of physiology. Cell physiology, Vol. 296, Issue 3, pp. C570-82, (2009) (PubMed).
-
-
- SLCO1B1 (Solute Carrier Organic Anion Transporter Family, Member 1B1 (SLCO1B1))
-
Alternative Name
- SLCO1B1
-
Background
- This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and leukotriene C4. This protein is also involved in the removal of drug compounds such as statins, bromosulfophthalein and rifampin from the blood into the hepatocytes. Polymorphisms in the gene encoding this protein are associated with impaired transporter function. [provided by RefSeq, Mar 2009].
-
NCBI Accession
- NM_006446, NP_006437
Target
-
You are here:
