Human SLCO1B3 cDNA Clone in Mammalian Expression Vector
This is a Solute Carrier Organic Anion Transporter Family, Member 1B3 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 3000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3380067
Supplier Product No.: sc113235
$955.35
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 21 to 22 Business Days
Quick Overview for Human SLCO1B3 cDNA Clone in Mammalian Expression Vector (ABIN3380067)
Gene
SLCO1B3
(Solute Carrier Organic Anion Transporter Family, Member 1B3 (SLCO1B3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc113235
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLCO1B3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Role of OATP transporters in steroid uptake by prostate cancer cells in vivo." in: Prostate cancer and prostatic diseases, (2016) (PubMed).
: "Mechanisms of pH-gradient driven transport mediated by organic anion polypeptide transporters." in: American journal of physiology. Cell physiology, Vol. 296, Issue 3, pp. C570-82, (2009) (PubMed).
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- SLCO1B3 (Solute Carrier Organic Anion Transporter Family, Member 1B3 (SLCO1B3))
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Alternative Name
- SLCO1B3
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Background
- This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of endogenous and xenobiotic compounds and plays a critical role in bile acid and bilirubin transport. Mutations in this gene are a cause of Rotor type hyperbilirubinemia. [provided by RefSeq, Feb 2012].
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NCBI Accession
- NM_019844, NP_062818
Target
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